normal human skin dermal fibroblast cell line  (PromoCell)

Journal: Cancer Management and Research

Article Title: Potential of Using New Indole- and Benzimidazo[1,2-C]quinazolines in Anticancer Therapy Based on Mesenchymal Stem Cells

doi: 10.2147/CMAR.S516593

Figure Lengend Snippet: Evaluation of the IC 50 values of compound A determined for ASC52-telo cells and HDFa cells in 48- and 72-hour cell cultures.

Article Snippet: Human skin fibroblast cell line (HDFa) (ATCC, PCS-201-012, Primary Dermal Fibroblast; Normal, Human, Adult) - a normal, adherent, skin cell line with research applications in responding to pathogens, skin aging, wound healing, gene delivery and skin diseases including scleroderma.

Techniques:

Journal: Cancer Management and Research

Article Title: Potential of Using New Indole- and Benzimidazo[1,2-C]quinazolines in Anticancer Therapy Based on Mesenchymal Stem Cells

doi: 10.2147/CMAR.S516593

Figure Lengend Snippet: Evaluation of the IC 50 values of compound B determined for ASC52-telo cells and HDFa cells in 48- and 72-hour cell cultures.

Article Snippet: Human skin fibroblast cell line (HDFa) (ATCC, PCS-201-012, Primary Dermal Fibroblast; Normal, Human, Adult) - a normal, adherent, skin cell line with research applications in responding to pathogens, skin aging, wound healing, gene delivery and skin diseases including scleroderma.

Techniques:

Journal: Scientific Reports

Article Title: Molecular imaging of aberrant crypt foci in the human colon targeting glutathione S-transferase P1-1

doi: 10.1038/s41598-017-06857-x

Figure Lengend Snippet: Correlation between GST activity and fluorescence intensity in various cancer cell lines. ( A ) Seven cancer cell lines (MKN45, HuH-28, HGC27, DLD1, CCK81, M7609, and NUGC-4) were incubated with DNAT-Me at 10 μM for 10 min and observed under fluorescence microscopy with excitation at 488 nm. Fluorescence intensity per cell in each cell line was quantitated using software provided by the manufacturer, as described in Materials and Methods. A primary normal adult human dermal fibroblast line (HDFa) was used as a negative control. ( B ) The cytosolic fraction was extracted from each cell line, and GST activity was measured using 1-chloro-2,4- dinitrobenzene and glutathione as substrates.

Article Snippet: Adult human dermal fibroblasts (HDFa), as a primary normal adult human dermal fibroblast cell line from skin, were purchased from Thermo Fisher Scientific Inc. (Wilmington, DE).

Techniques: Activity Assay, Fluorescence, Incubation, Microscopy, Software, Negative Control